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KMID : 0385219970070030037
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Korean Journal of Gerontology
1997 Volume.7 No. 3 p.37 ~ p.41
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Nitric Oxide and Bone Formation in Osteoblasts
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Kang Jang-Sook
Kim Hyung-Ryong
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Abstract
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Recently, nitric oxide(NO), which functions as a neurotransmitter, endothelial-derived relaxation factor and immune effector molecule, is known to be implicated in the metabolism of bone, especially as a mediator of cytokine effects on the remodelling of bone tissue. In this study we examine whether NO affects the osteoblast activation of primary mouse osteblast-like and osteosarcoma ROS 17/2.8 cells released NO when stimulated with the combination of interleukin-1β, tumour necrosis factor-α and interferon-γ. Sodium nitroprusside, a donor of nitric oxide, increased the activity of alkaline phosphatase in ROS 17/2.8 cells as well as the number of calcified nodule formations in primary mouse osteoblast-like cells. These results indicated that NO appears to be an important regulatory molecule in the processes of bone remodelling.
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KEYWORD
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Osteoblast, Nitric Oxide, Alkaline phophatase, Bone nodule formation
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